KOMISJA CHEMII I FIZYKI
W BIOLOGII I MEDYCYNIE
ODDZIAŁU POLSKIEJ AKADEMII
NAUK WE WROCŁAWIU
na posiedzenie naukowe, które odbędzie się w
poniedziałek 15 maja 2023 r. o godz. 16.30
w siedzibie Oddziału Polskiej Akademii Nauk we Wrocławiu,
ul. Podwale 75 (sala konferencyjna – parter).
Podczas posiedzenia wykład pt.:
"Specific or not specific, that is the question»: an overview on gal-1 - GM1 interaction"
Federica Scollo, PhD student
Charles University of Prague- Department of Physical and Macromolecular Chemistry
Galectins are a ubiquitous family of galactose-binding proteins, they possess one or more β-sandwich carbohydrate recognition domain/s (CRD/s) by which they interact with molecules having a galactoside moiety. Nowadays, fourteen human galectins have been discovered, commonly classified into three different types, according to their different architecture. Among all of them, galectin-1 is of great interest, being implicated in T-cells communication. Gal-1 is believed to express its function by binding specifically the monosialotetrahexosylganglioside (GM1). The above-mentioned binding has been hypothesized and indirectly shown in previous works, either employing too sophisticated or non-physiologically relevant systems, such as neuroblastoma cells or glycodendrimers, respectively. However, whether this binding is specific and what is the dissociation constant (Kd), as well as other thermodynamic parameters, are still open questions. In this work, we investigate the interaction of galectin-1 with different types of physiologically relevant model membranes containing GM1, i.e., free-standing and supported Large Unilamellar Vesicles (LUVs), Giant Unilamellar Vesicles (GUVs), as well as with living cells. We employed a combination of computational (Molecular Dynamic Simulations) and experimental different techniques, both fluorescence-based, i.e. Förster Resonance Energy Transfer (FRET) and confocal Fluorescence Microscopy combined with Quartz Crystal Microbalance with Dissipation Monitoring (QCM-D) and Isothermal Titration Calorimetry, that are label-free techniques. To the best of our knowledge, our data show for the first time that galectin-1 is interacting with model lipid membranes and, more importantly, the data suggest a specific interaction with GM1.
Chemii i Fizyki w Biologii i Medycynie
Dr hab. Hanna Pruchnik